Upload your .ab1 files by dragging them here or clicking "Browse...".
For paired-end assembly, ensure file pairs have identical names except for the "_Fwd.ab1" and "_Rev.ab1" suffixes.
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Trimmed Sequences
Trimming Report
BLAST Result
Welcome to Niangao!
Niangao is an all-in-one tool for processing Sanger sequencing results (.ab1 files).
Extracts continuous high-quality sequences in FASTA format from your .ab1 files, enabling downstream analysis.
Assembly & BLAST
Assembles paired "_Fwd.ab1" and "_Rev.ab1" files into contigs using CAP3.
Provides either the successfully assembled contig or the longest clean input (Fwd or Rev) sequence.
Allows you to BLAST your sequences, choose the database, and set the number of hits per page.
Contact & Code
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